Gas Chromatography of Aqueous Saponin Extract of Heinsia crinata Leaves.

T.F.B. Miikue-Yobe, J. O .Akaninwor, A. .A. Uwakwe


The quantization and separation of bioactive phytochemicals by chromatographic methods is one of the procedures for drug testing and formulation.The aqueous saponin extract of Heinsia crinata was derivatized and used to screen for the presence and identification of bioactive fractions present in the aqueous medium with the view to ascertaining its antidiabetic property. The chromatogram revealed the presence of eighteen types of saponin fractions present in the extract. The quantitative data shows that Sapogenin (93.60mg/100g) was the highest, followed by Saponine (28.65mg/100g), Neochlorogenin (11.72mg/100g), Diosgenin (9.86mg/100g) and the least been saponoside A (1.20mg/100g) amount of the extract. The fractions with the higher values are known to possess antidiabetic properties. This finding therefore supports the use of saponin extract of Heinsia crinata in the treatment of diabetes mellitus and so is a potential source for the development of such hypoglycaemic agents.

Keywords- Heinsia crinata, Saponine, Sapogenin, Diosgenin, Gas Chromatograpy, Quantization.

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