FT-IR. CHROMATOGRAPHIC AND ANTIMICROBIAL ACTIVITIES OF SOME POLYPHENOLICS FROM ACETONE EXTRACT OFTHE LEAVES OF CHENOPODIUM AMBROSIOIDES LINN.

A. M. Lohdip, S. T. Yakubu, C. O. Kelechi

Abstract


The leaf powder of Chenopodium ambrosioides (Linn) was extracted using 70% acetone. Gel column chromatography (CC) was carried out using toluene, acetone and formic acid, respectively, as the eluting solvents. CC fractions were monitored using thin layer chromatography (TLC) with acetone-toluene-formic acid (6:6:1) as the solvent system and were combined based on similarities of Rf values; the combined fractions were tested for polyphenols and FT-IR, as well as High power liquid chromatography (HPLC) analyses for tannins was performed on them. Fractions that tested positive for tannins were tested for antimicrobial activities on Escherichia Coli, Salmonella Typhi, Staphylococcus aureus and Candida albicans. The TLC analysis on the crude extract gave 8 spots ( Rf 0.88, 0.10, 0.16, 0.40, 0.41, 0.50,0.56 and 0.60). The CC analysis gave 10 combined fractions based on similarities of Rf values, out of which 5 (labelled F39, F48, F50, F55 and F68 recoded YFL, YJL, YDL,YEL and YLL, respectively) tested positive for polyphenolics. The FT-IR spectrum revealed broad and sharp peak due to polymeric OH stretch at 3333cm-1; C-H stretch of aromatic ring at 2931cm-1;. there bands at 1931 cm-1, 1718 cm-1 and 1619 cm-1 which  could be attributed to C=C stretching of  ketenes, C=O stretching of ketones and C=C stretch of aromatic system.  HPLC showed that the five fractions were hydrolysable tannins (YFL and YEL at 280 nm, YDL at 272 nm, YJL and YLL at 273 nm). Both TLC and HPLC showed that fraction F39 was tannic acid. They were all active on E. Coli, S. typhi, S.  aureus and C. albicans.

KEY WORDS: Chromatography, Gram negative, Gram positive and Yeast

 


Full Text:

PDF

Refbacks

  • There are currently no refbacks.


Chemical Society of Nigeria