Characterization and Antimicrobial activity of Copper nanoparticles synthesized using Anthocyanin-rich aqueous extracts of Costus afer

A. Hamilton-Amachree, O. O. Abosede


Copper nanoparticles (CuNPs) were synthesized using anthocyanin-rich aqueous extracts of Costus afer flowers as reducing and capping agents. The reduction process of Cu2+ to Cu0 was determined by the change of colour from red to brown after the addition of copper nitrate to the aqueous extract of Costus afer flowers. The formation of copper nanoparticles was monitored by UV and IR spectrophotometry. Simple qualitative tests were carried out on the Costus afer flower extract to determine the presence of anthocyanins and other phytochemicals which could be responsible for the reduction process.  The size and morphology of the CuNPs were further assessed through XRD and SEM. The CuNPs showed spherical morphology with an average size of 1.3 nm. Thermogravimetric analysis of the nanoparticles revealed that the bioactive capping agents decomposes between 220 and 280 °C and is 51% of the mass of the nanoparticles. The antimicrobial activities of the nanoparticles were evaluated against some pathogenic microorganisms. 

Keywords:  Synthesis, Copper nanoparticles, Costus afer, FTIR, XRD, SEM and TGA analyses, Antimicrobial activity.    

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