ANALYSIS OF ANTIBIOTICS IN POULTRY WASTEWATER AND DROPPINGS USING SOLID PHASE EXTRACTION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

T.O. Oluseyi, A.O. Oyeyiola, B. Rabiu, N. Mbadiwe, K.O. Olayinka, B.O. Silva

Abstract


A method was developed for the extraction, preconcentration and analysis of two antibiotics Amoxicillin (AMOX) and Oxytetracycline (OXY)) in poultry waste water and droppings. The method was based on solid phase extraction (SPE) and high performance liquid chromatography with ultraviolet detection (HPLC-UV). Optimization of the chromatographic conditions and the conditioning of the SPE sorbents were carried out to obtain the optimal condition for the analysis. Three eluting solvents were tested to establish the optimum conditions for the SPE procedure and pH values of samples were varied prior to HPLC analysis. Methanol gave the best percentage recoveries of 67.61% and 30.98%, followed by acetone with recoveries of 66.32% and 24.31% while acetonitrile had the poorest percentage recoveries of 64.23% and 19.05% for Amoxicillin and Oxytetracycline respectively. In the analysis of poultry waste water and droppings, the matrix components in the samples reduced the extraction efficiency leading to a decrease in the percentage recoveries of samples. The decrease in percentage recoveries obtained for the spiked poultry waste water samples followed the same trend as the standard with methanol giving the best percentage recoveries of 60.27% and 23.04%, followed by acetone with recoveries of 45.37% and 21.65% while acetonitrile gave the poorest percentage recoveries of 29.12% and 16.21% for Amoxicillin and Oxytetracycline respectively.  The non-spiked poultry wastewater at pH 2.5 had the highest concentration of 19.00 ppm for the AMOX, while at pH 7.0, the lowest concentration of 10.04 ppm was observed, while for the OXY, the only detectable concentration was at pH 9.0, with a value of 6.16ppm.

 Keywords: Amoxicillin, antibiotics, HPLC, Oxytetracycline, poultry, SPE, wastewater


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Chemical Society of Nigeria