CHEMICAL CONSTITUENTS AND LIPASE INHIBITORY ACTIVITIES OF SOLVENT FRACTIONS OF ANTHOCLEISTA VOGELII STEM BARK

O. S. Balogun, Zhiqiang Liu

Abstract


Stem bark of Anthocleista vogelii was extracted and fractionated to give hexane and methanol fractions. The solvent fractions were silylated and analysed using GC-MS, thereafter, the fractions were investigated for in vitro inhibition of pancreatic lipase enzyme. Fourteen compounds were identified from the hexane fraction, with free fatty acids and triterpenoids accounting for 60.18% and 20.75% respectively. Monosaccharides, particularly mannose (49.10%) were predominant in methanol fraction. The lipase inhibitory assay showed a concentration-dependent activity for hexane fraction while the activity of methanol fraction was largely independent of concentration. At 400 µg/mL, hexane and methanol fractions demonstrated anti-lipase activities of 91.42% and 54.70% respectively. The high inhibitory potency of stem bark extract of A. vogelii on pancreatic lipase could be explored further in the development of safe therapy for obesity. 

Keywords: Anthocleista vogelii; silylation; pancreatic lipase; obesity; triterpenoids; GC-MS.


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Chemical Society of Nigeria