OPTIMIZATION OF PROCESS PARAMETERS FOR THE EXTRACTION OF BIOACTIVE COMPOUNDS FROM TETRAPLEURA TETRAPTERA USING D-OPTIMAL DESIGN.
AbstractPlants are the traditional sources for many chemicals used as pharmaceutical biochemical, fragrances, food colours and flavours in different countries. Medicinal plants such as Tetrapleura Tetraptera are at great interest to the researcher in the field of biotechnology, as most of the drug industries depend on them for the production of pharmaceutical compounds. In this work, the effects of several ex traction variables on the yield of bioactive compounds in Tetrapleura Tetraptera were investigated. The soxhlet extraction process was designed employing D-Optimal designs. The process variables considered and their range of values are extraction temperature (78 - 90oC), particle size (1.00 - 5.00mm) and extraction time (10 - 50mins). The bioactive compounds in Tetrapleura Tetraptera extracts were identified using Gas chromatography/ Mass spectrometer (GC-MS) technique. A cubic model was obtained to predict the extraction yield as a function of extraction temperature, particle size and extraction time. The study revealed that the yield of the bioactive compounds increased with extraction time, decreased with particle size and increased with temperature. The optimum yield of the bioactive compounds of over 73% was obtained at an extraction temperature of 89.45oC, particle size of 1.51mm and extraction time of 36.23mins. High R2 value of 0.9993 and the correlation between the adjusted and predicted R2 values of 0.9973 and 0.9875 respectively showed that D-Optimal design is highly for the extraction of essential bioactive components from medicinal plants. The GC-MS analysis revealed the presence of various bioactive compounds such as acetic acid, glyceraldhyde, piperazine, octodrine, glycidol and D-Fructose in the extract of Tetrapleura Tetraptera. Keywords: Tetrapleura tetraptera, extraction, D-optimal design, optimization.