PHYTOCHEMICAL , ANTIMICROBIAL AND ANTI-INFLAMMATION ANALYSES OF THE FRACTIONATED METHANOL EXTRACT OF THE SEEDS OF TETRACARPIDIUM CONOPHORUM
AbstractPlants contain pharmacologically potent phytochemicals some of which have ground breaking records in the prevention and treatment of diseases in man and animals. Activity guided fractionation of the crude methanol extract of the seeds of Tetracarpidium conophorum using hexane, ethyl acetate, butanol and distilled water were carried out. The concentrated crude extract and fractions were subjected to phytochemical analysis .The fractions were further subjected to anti-microbial and anti-inflammation analyses, using Kirby-Bauer disc diffusion and Xylene induced mouse ear edema methods respectively. The organisms used for the anti-microbial analysis were Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudonomas aeruginosa, Klebsiella pneumonia, Bacillus subtilis, Candida albicans and Aspergillus flavus. The result of the phytochemical analysis showed the presence of alkaloids, flavonoids, saponins and tannins. Glycosides and steroids were absent. Results of anti-microbial analysis showed hexane and butanol fractions to have mild concentration dependent activity on Bacillus subtilis at concentrations of 50, 100, 200 and 300µg/mL. Ethyl acetate fraction had the highest activity on Bacillus subtilis at 300 µg/mL. The aqueous fraction had no activity on any of the organisms. The anti-inflammation analysis showed that the butanol fraction had the highest activity of 85.71% at 250 µg/mL. The ethyl acetate fraction had the second highest activity of 46.94% at 250 µg/mL. This shows that the extracts of the seeds of Tetracarpidium conophorum will be very useful in the production of drugs and nutraceuticals to tackle anti-inflammation and related diseases in man and animals. Key words: Tetracarpidium conophorum, fractionation, phytochemical, anti-inflammation, anti-microbial, ear edema.