CHEMICAL CONSTITUENTS AND AN ANTIMICROBIAL ASSAY OF METHANOL ROOT AND LEAVES EXTRACTS OF Hippocratea indica Willd.

Authors

  • O. S. Sojinu
  • D. I. Akinloye
  • A. M. Mosaku
  • A. Ta. Adebusuyi
  • D. Oduntan
  • A. E. Afolabi
  • M. K. O. Arifalod

DOI:

https://doi.org/10.46602/jcsn.v67i3.753

Abstract

The plant Hippocratea indica is reported in ethnomedicine for treating various illnesses like guinea worm infestation and respiratory troubles. Hippocratea indica root and leaves were extracted respectively using methanol. The preliminary phytochemical screening of the respective crude methanol root and leaves extracts of Hippocratea indica revealed the presence of alkaloids, flavonoids and glycoside. At the same time, phlobatannins was found only in the root. Column chromatography of the fractionated extract was carried out to get saturated, aromatic and non-polar fractions. The methanol crude extract of Hippocratea indica Willd indicates that the root and leaves extracts have high antimicrobial activities with higher sensitivity for the gram-negative bacteria than the gram-positive ones. The first fraction's gas chromatography-mass spectrometry (GC-MS) analysis was performed. Forty-six compounds were identified in the methanol root extract, while thirteen were identified in the leaf extract through GC-MS analysis. The most abundant phytochemical was m-pyrol (23.73 %), followed by fumaric acid (8.59 %) and squalene (6.03 %) in the root extracted, while trans-geranylgeraniol (30.56 %), 2,6-ditert-butyl-4-[(2-octadecyloxycarbonyl) ethyl] phenol (22.51 %) and squalene (20.18 %) are in the leaves extract. These most abundant phytochemicals might be responsible for methanol root's antibacterial and anti-inflammatory activity and leave extract of Hippocratea indica.

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Published

2022-06-24

How to Cite

Sojinu, O. S., Akinloye, D. I. ., Mosaku, A. M., Adebusuyi, A. T. ., Oduntan, D. ., Afolabi, A. E. ., & Arifalod, M. K. O. (2022). CHEMICAL CONSTITUENTS AND AN ANTIMICROBIAL ASSAY OF METHANOL ROOT AND LEAVES EXTRACTS OF Hippocratea indica Willd . Journal of Chemical Society of Nigeria, 47(3). https://doi.org/10.46602/jcsn.v67i3.753