ANTI-INFLAMMATORY ACTIVITY OF DEINBOLLIA PINNATA LEAVES WITH ISOLATION OF TRITERPENOIDS

Abstract

Inflammation is the secondary signal from tissue/cell injury or damage. Most often have increased temperature, and blood flow and are characterized by pain, coloration and swelling. Leaves of Deinbollia pinnata have been into use for anti-inflammatory diseases in rural populated areas of African countries by barefoot doctors. The leaves of D. pinnata investigated showed higher anti-inflammatory with methanol, ethyl acetate and n-hexane in order of (80%, 50% and 14%) at 50 µL for bovine serum albumin assay; and methanol, ethyl acetate and n-hexane (85%, 57%, and 20%) at 40 µL using egg albumin assay. The result showed that the methanolic fraction with less quantity of yield has the highest anti-inflammatory potential compared to other fractions indicating the presence of polar unidentified compounds. Seven (7) known triterpenes were reported using combined chromatographic separation from ethyl acetate fraction which showed the highest yield of extract. These include squalene (1) stigmasterol (2), stigmasta-5,22-diene-3-ol acetate (3), ?-sitosterol (4), lupeol (5), taraxasterol (6), and betulinic acid (7) from aerial parts. Their structures were elucidated using HR-ESIMS, 1D NMR compared with existing data which confirmed their uses in African traditional medicine as anti-inflammatory and antioxidant.