In Vitro Anti-Inflammation Studies of Jakaranda mimosifoliaExtract Using Protein Denaturation Technique.

Abstract

The present study investigates the anti-inflammation potential of Jacaranda mimosifolia, which is reported in traditional medicine. Microwave energy was used for the extraction of plant material and, the anti-inflammatory activity was evaluated using protein denaturation method. In-vitro anti inflammation test on different concentrations of the extracts solution was conducted. The highest percentage inhibition of protein denaturation of the lowest concentration (31.25 µg/ml) of the extracts was observed as 86. 21 % in the chloroform extract (CE), which is highly significant and comparable to that of  the standard drug (Diclofenac) shown as 82.76 %. The hexane extract (HE) and ethylacetate extract (EE) both inhibited protein denaturation at 68.97 % and that of methanol extract (ME) was observed as 68.97 %. The result of our investigation reveals that Jacaranda mimosifolia has potentials to be explored in the search for a potent anti-inflammatory drug from natural origin. Therefore our studies support the use of the plant in the treatment of inflammation in traditional medicine. Key-words:Jacaranda mimosifolia, Protein denaturation, Anti-inflammatory, Microwave Assisted Extraction.